Molecular analysis of herbivore-induced condensed tannin synthesis: cloning and expression of dihydroflavonol reductase from trembling aspen (Populus tremuloides).
Identifieur interne : 004558 ( Main/Exploration ); précédent : 004557; suivant : 004559Molecular analysis of herbivore-induced condensed tannin synthesis: cloning and expression of dihydroflavonol reductase from trembling aspen (Populus tremuloides).
Auteurs : Darren J. Peters [Canada] ; C Peter ConstabelSource :
- The Plant journal : for cell and molecular biology [ 0960-7412 ] ; 2002.
Descripteurs français
- KwdFr :
- Alcohol oxidoreductases (composition chimique), Alcohol oxidoreductases (génétique), Alcohol oxidoreductases (métabolisme), Alignement de séquences (MeSH), Animaux (MeSH), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Facteurs temps (MeSH), Gènes de plante (génétique), Induction enzymatique (MeSH), Maladies des plantes (génétique), Maladies des plantes (parasitologie), Papillons de nuit (physiologie), Populus (enzymologie), Populus (génétique), Populus (métabolisme), Populus (parasitologie), Régulation de l'expression des gènes végétaux (MeSH), Similitude de séquences d'acides aminés (MeSH), Structure moléculaire (MeSH), Séquence d'acides aminés (MeSH), Tanins hydrolysables (composition chimique), Tanins hydrolysables (métabolisme).
- MESH :
- composition chimique : Alcohol oxidoreductases, Tanins hydrolysables.
- enzymologie : Populus.
- génétique : Alcohol oxidoreductases, Gènes de plante, Maladies des plantes, Populus.
- métabolisme : Alcohol oxidoreductases, Populus, Tanins hydrolysables.
- parasitologie : Maladies des plantes, Populus.
- physiologie : Papillons de nuit.
- Alignement de séquences, Animaux, Clonage moléculaire, Données de séquences moléculaires, Facteurs temps, Induction enzymatique, Régulation de l'expression des gènes végétaux, Similitude de séquences d'acides aminés, Structure moléculaire, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Alcohol Oxidoreductases (chemistry), Alcohol Oxidoreductases (genetics), Alcohol Oxidoreductases (metabolism), Amino Acid Sequence (MeSH), Animals (MeSH), Cloning, Molecular (MeSH), Enzyme Induction (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (genetics), Hydrolyzable Tannins (chemistry), Hydrolyzable Tannins (metabolism), Molecular Sequence Data (MeSH), Molecular Structure (MeSH), Moths (physiology), Plant Diseases (genetics), Plant Diseases (parasitology), Populus (enzymology), Populus (genetics), Populus (metabolism), Populus (parasitology), Sequence Alignment (MeSH), Sequence Homology, Amino Acid (MeSH), Time Factors (MeSH).
- MESH :
- chemical , chemistry : Alcohol Oxidoreductases, Hydrolyzable Tannins.
- chemical , genetics : Alcohol Oxidoreductases.
- chemical , metabolism : Alcohol Oxidoreductases, Hydrolyzable Tannins.
- enzymology : Populus.
- genetics : Genes, Plant, Plant Diseases, Populus.
- metabolism : Populus.
- parasitology : Plant Diseases, Populus.
- physiology : Moths.
- Amino Acid Sequence, Animals, Cloning, Molecular, Enzyme Induction, Gene Expression Regulation, Plant, Molecular Sequence Data, Molecular Structure, Sequence Alignment, Sequence Homology, Amino Acid, Time Factors.
Abstract
In order to study condensed tannin synthesis and its induction by herbivory, a dihydroflavonol reductase (DFR) cDNA was isolated from trembling aspen (Populus tremuloides). Bacterial overexpression demonstrated that this cDNA encodes a functional DFR enzyme, and Southern analysis revealed that DFR likely is a single-copy gene in the aspen genome. Aspen plants that were mechanically wounded showed a dramatic increase in DFR expression after 24 h in both wounded leaves and unwounded leaves on wounded trees. Feeding by forest tent caterpillar (Malacosoma disstria) and satin moth (Leucoma salicis) larvae, and treatment with methyl jasmonate, all strongly induced DFR expression. DFR enzyme activity was also induced in wounded aspen leaves, and phytochemical assays revealed that condensed tannin concentrations significantly increased in wounded and systemic leaves. The expression of other genes involved in the phenylpropanoid pathway were also induced by wounding. Our findings suggest that the induction of condensed tannins, compounds known to be important for defense against herbivores, is mediated by increased expression of DFR and other phenylpropanoid genes.
DOI: 10.1046/j.1365-313x.2002.01458.x
PubMed: 12472686
Affiliations:
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Bacterial overexpression demonstrated that this cDNA encodes a functional DFR enzyme, and Southern analysis revealed that DFR likely is a single-copy gene in the aspen genome. Aspen plants that were mechanically wounded showed a dramatic increase in DFR expression after 24 h in both wounded leaves and unwounded leaves on wounded trees. Feeding by forest tent caterpillar (Malacosoma disstria) and satin moth (Leucoma salicis) larvae, and treatment with methyl jasmonate, all strongly induced DFR expression. DFR enzyme activity was also induced in wounded aspen leaves, and phytochemical assays revealed that condensed tannin concentrations significantly increased in wounded and systemic leaves. The expression of other genes involved in the phenylpropanoid pathway were also induced by wounding. Our findings suggest that the induction of condensed tannins, compounds known to be important for defense against herbivores, is mediated by increased expression of DFR and other phenylpropanoid genes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12472686</PMID><DateCompleted><Year>2004</Year><Month>01</Month><Day>16</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>06</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0960-7412</ISSN><JournalIssue CitedMedium="Print"><Volume>32</Volume><Issue>5</Issue><PubDate><Year>2002</Year><Month>Dec</Month></PubDate></JournalIssue><Title>The Plant journal : for cell and molecular biology</Title><ISOAbbreviation>Plant J</ISOAbbreviation></Journal><ArticleTitle>Molecular analysis of herbivore-induced condensed tannin synthesis: cloning and expression of dihydroflavonol reductase from trembling aspen (Populus tremuloides).</ArticleTitle><Pagination><MedlinePgn>701-12</MedlinePgn></Pagination><Abstract><AbstractText>In order to study condensed tannin synthesis and its induction by herbivory, a dihydroflavonol reductase (DFR) cDNA was isolated from trembling aspen (Populus tremuloides). Bacterial overexpression demonstrated that this cDNA encodes a functional DFR enzyme, and Southern analysis revealed that DFR likely is a single-copy gene in the aspen genome. Aspen plants that were mechanically wounded showed a dramatic increase in DFR expression after 24 h in both wounded leaves and unwounded leaves on wounded trees. Feeding by forest tent caterpillar (Malacosoma disstria) and satin moth (Leucoma salicis) larvae, and treatment with methyl jasmonate, all strongly induced DFR expression. DFR enzyme activity was also induced in wounded aspen leaves, and phytochemical assays revealed that condensed tannin concentrations significantly increased in wounded and systemic leaves. The expression of other genes involved in the phenylpropanoid pathway were also induced by wounding. Our findings suggest that the induction of condensed tannins, compounds known to be important for defense against herbivores, is mediated by increased expression of DFR and other phenylpropanoid genes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Peters</LastName><ForeName>Darren J</ForeName><Initials>DJ</Initials><AffiliationInfo><Affiliation>Department of Biological Sciences, University of Alberta, Edmonton, Canada T6G 2E9.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Constabel</LastName><ForeName>C Peter</ForeName><Initials>CP</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AY147903</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. 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